Published in bioRxiv, currently in review, 2024
Dissecting the lytic phage lifecycle by imaging phage infections at the single virion and cell level in the mother machine.
Recommended citation: Wedd, Charlie., et al. (2024). "Single-cell imaging of the lytic phage life cycle in bacteria." bioRxiv (in review)
Download Paper | Download Bibtex
Published in npj Imaging, 2024
Analysing optical artefacts affecting quantification of single-cell parameters using virtual microscopy and bioimage analysis of targetted experiments.
Recommended citation: Hardo, Georgeos., et al. (2024). "Quantitative microbiology with widefield microscopy: navigating optical artefacts for accurate interpretations." npj Imaging
Download Paper | Download Bibtex
Published in BMC Biology, 2022
A tool to generate synthetic training data of bacteria under the microscope to train highly accurate and precise segmentation and tracking models.
Recommended citation: Hardo, Georgeos., et al. (2022). "Synthetic Micrographs of Bacteria (SyMBac) allows accurate segmentation of bacterial cells using deep neural networks." BMC Biology. 20, 263.
Download Paper | Download Bibtex
Published in Essays in Biochemistry, 2021
Here we look at the issues surrounding measurement of single-cell gene expression in bacteria growing in microfluidic devices such as the mother machine.
Recommended citation: Hardo, Georgeos., Bakshi, Somenath. (2021). "Challenges of analysing stochastic gene expression in bacteria using single-cell time-lapse experiments." Essays in Biochemistry. 65(1).
Download Paper | Download Bibtex