Published in Nature Microbiology, 2025
This paper shows that porin permeability in E. coli is controlled by periplasmic H+ and K+ concentrations, linking metabolism to antibiotic resistance.
Recommended citation: Caño Muñiz, S.E., Trigg, S., Hardo, G. et al. (2025). "Metabolic control of porin permeability influences antibiotic resistance in Escherichia coli." Nat Microbiol 10, 3202–3214.
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Published in The EMBO Journal, 2025
This paper reveals how glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli.
Recommended citation: Thappeta, Y., Cañas-Duarte, S.J., Wang, H. et al. (2025). "Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli." EMBO J 44, 7434–7476.
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Published in bioRxiv, currently in review, 2024
Dissecting the lytic phage lifecycle by imaging phage infections at the single virion and cell level in the mother machine.
Recommended citation: Wedd, Charlie., et al. (2024). "Single-cell imaging of the lytic phage life cycle in bacteria." bioRxiv (in review)
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Published in npj Imaging, 2024
Analysing optical artefacts affecting quantification of single-cell parameters using virtual microscopy and bioimage analysis of targetted experiments.
Recommended citation: Hardo, Georgeos., et al. (2024). "Quantitative microbiology with widefield microscopy: navigating optical artefacts for accurate interpretations." npj Imaging
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Published in BMC Biology, 2022
A tool to generate synthetic training data of bacteria under the microscope to train highly accurate and precise segmentation and tracking models.
Recommended citation: Hardo, Georgeos., et al. (2022). "Synthetic Micrographs of Bacteria (SyMBac) allows accurate segmentation of bacterial cells using deep neural networks." BMC Biology. 20, 263.
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Published in Essays in Biochemistry, 2021
Here we look at the issues surrounding measurement of single-cell gene expression in bacteria growing in microfluidic devices such as the mother machine.
Recommended citation: Hardo, Georgeos., Bakshi, Somenath. (2021). "Challenges of analysing stochastic gene expression in bacteria using single-cell time-lapse experiments." Essays in Biochemistry. 65(1).
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